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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Ras-related protein Rab-10, Rab10
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Ras- related protein Rab- 10, Rab10 ELISA KIT
Product Name:Mouse Ras- related protein Rab- 10, Rab10 ELISA KIT
Packing:96T
Catalog No.:ELI-14073m
Gene Name:Mouse Rab10
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Rab10
Alternative Name:Mouse Ras-related protein Rab-10, Rab10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ras- related protein Rab- 10, Rab10 ELISA KIT allows for the in vitro quantitative determination of Mouse Rab10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ras- related protein Rab- 10, Rab10 ELISA KIT has been pre-coated with an Mouse Ras-related protein Rab-10, Rab10 antibody specific to Mouse Rab10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rab10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rab10 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rab10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Rab proteins are small Ras-like GTPases that regulate vesicular trafficking events in the cell. More than 50 Rabs have been described in mammalian cells (1 ), each with a specific subcellular localization reflecting the functional
PNAS:RAS 抑制剂又添一员,新型蛋白模拟物或为癌症治疗
13、RAB10、RAB14、RAB18、RAB5C 和 RAP1B)。序列分析表明,在 Sos 螺旋发夹结合区富集的 Ras 相关蛋白和其他 G 蛋白之间存在高度的序列相似性。图片来源:PNAS总的来说,该研究开发了一种 Sos 蛋白模拟物,它结合野生型和突变型 Ras,并对致癌 Ras 细胞具有选择性毒性作用。这种模拟物将为开发高 Ras 突变型癌症靶向抑制剂提供坚实的理论依据。延伸阅读该研究是同一团队十年前报道的跟进研究。图片来源:ScienceDirect 截图2011 年,Paramjit S
人Dickkopf related protein 1 (DKK-1)ELISA试剂盒 说明书
人 Dickkopf related protein 1 ( DKK-1 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 DKK-1 单抗包被于酶标板上,标准品和样品中的 DKK-1与单抗结合,加入生物素化的抗人 DKK-1 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入
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