Keratin, type II cytoskeletal
80, Krt80 ELISA KIT/ Mouse Keratin, type II cytoskeletal 80, Krt80 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-14065m
- 2025年12月28日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Keratin, type II cytoskeletal 80, Krt80
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Keratin, type II cytoskeletal 80, Krt80 ELISA KIT
Product Name:Mouse Keratin, type II cytoskeletal 80, Krt80 ELISA KIT
Packing:96T
Catalog No.:ELI-14065m
Gene Name:Mouse Krt80
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Krt80
Alternative Name:Mouse Keratin, type II cytoskeletal 80, Krt80
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Keratin, type II cytoskeletal 80, Krt80 ELISA KIT allows for the in vitro quantitative determination of Mouse Krt80 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Keratin, type II cytoskeletal 80, Krt80 ELISA KIT has been pre-coated with an Mouse Keratin, type II cytoskeletal 80, Krt80 antibody specific to Mouse Krt80 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Krt80 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Krt80 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Krt80 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Transient Transfections and Heterokaryons as Tools for the Analysis of Keratin IF Dynamics
All eukaryotic cells contain three distinct cytoskeletal networks: microtubules, microfilaments, and intermediate filaments (IF). In epithelial cells in vivo, the IF cytoskeleton is composed of heteropolymers of type I (acidic) and type II
Summary The intermediate filament (IF) cytoskeleton of mammalian epithelia is generated from pairs of type I and type II keratins that are encoded by two large gene families, made up of 54 genes in humans and the mouse
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
, the cell pellet can be snap-frozen in liquid nitrogen and stored at -80°C until use. Restriction Enzyme Digestion and Intramolecular Ligation 12. Resuspend the pellet in 500 µL of 1.14X restriction enzyme buffer (appropriate
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