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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Melanoma inhibitory activity protein 3, Mia3
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Melanoma inhibitory activity protein 3, Mia3 ELISA KIT
Product Name:Mouse Melanoma inhibitory activity protein 3, Mia3 ELISA KIT
Packing:96T
Catalog No.:ELI-14026m
Gene Name:Mouse Mia3
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Mia3
Alternative Name:Mouse Melanoma inhibitory activity protein 3, Mia3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Melanoma inhibitory activity protein 3, Mia3 ELISA KIT allows for the in vitro quantitative determination of Mouse Mia3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Melanoma inhibitory activity protein 3, Mia3 ELISA KIT has been pre-coated with an Mouse Melanoma inhibitory activity protein 3, Mia3 antibody specific to Mouse Mia3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mia3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mia3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mia3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Differential Display to Define Molecular Markers and Genes That Mediate Malignancy
S24 (7 ), 7. Genes for the histocompatibility antigen (HLA-DR), laminin B2, melanoma inhibitory activity, and tissue inhibitor metalloproteinase-3 (8 ), 8.
α response, we used two human-tumor-derived cell lines: the melanoma line ME-15 (10 ) and the hepatoma line HuH7 (11 ). We have chosen these cell lines as models, because we have a good understanding of the IFNα responses at the mRNA and the protein levels
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
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