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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
MKL
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse MKL/myocardin- like protein 1, Mkl1 ELISA KIT
Product Name:Mouse MKL/myocardin- like protein 1, Mkl1 ELISA KIT
Packing:96T
Catalog No.:ELI-14016m
Gene Name:Mouse Mkl1
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Mkl1
Alternative Name:Mouse MKL/myocardin-like protein 1, Mkl1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse MKL/myocardin- like protein 1, Mkl1 ELISA KIT allows for the in vitro quantitative determination of Mouse Mkl1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse MKL/myocardin- like protein 1, Mkl1 ELISA KIT has been pre-coated with an Mouse MKL/myocardin-like protein 1, Mkl1 antibody specific to Mouse Mkl1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mkl1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mkl1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mkl1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验,or write to the author. Identification of Tissue-Specific MicroRNAs from Mouse http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VRT-45X0D1F-N&_user=440026&_coverDate=04%2F30%2F2002&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c
目前自动洗板机应用比较广泛,凡是具有酶联免疫吸附技术(ELISA)的酶反应板均可使用。应用范围有:肿瘤标记物的检测、传染性疾病的检查、人类免疫缺陷病毒(HIV)抗体的检测、沙眼衣原体感染的血清学检查和TORCH感染的血清学检查等。应用ELISA完成这些方面的检测后,使用先进的全自动洗板机进行洗板,以保证测试的准确性和提高实验的可靠性。 自动洗板机是进行酶联免疫实验时,检测各种感染性病毒所必备的设备之一,其作用是通过清洗,实现酶联免疫实验过程中结合相与游离相的分离。当前,酶标洗板
of complex and protein alone with the naked eye or a light microscope, crystals of covalently Cy5?labeled DNA can be directly used for X?ray diffraction data collection. Curr. Protoc. Nucleic Acid Chem. 46:7.15.1?7.15.8. © 2011 by John Wiley & Sons
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