Myocyte-specific enhancer fact
or 2B, Mef2b ELISA KIT/ Mouse Myocyte-specific enhancer factor 2B, Mef2b ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-13966m
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Myocyte-specific enhancer factor 2B, Mef2b
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Myocyte- specific enhancer factor 2B, Mef2b ELISA KIT
Product Name:Mouse Myocyte- specific enhancer factor 2B, Mef2b ELISA KIT
Packing:96T
Catalog No.:ELI-13966m
Gene Name:Mouse Mef2b
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Mef2b
Alternative Name:Mouse Myocyte-specific enhancer factor 2B, Mef2b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Myocyte- specific enhancer factor 2B, Mef2b ELISA KIT allows for the in vitro quantitative determination of Mouse Mef2b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Myocyte- specific enhancer factor 2B, Mef2b ELISA KIT has been pre-coated with an Mouse Myocyte-specific enhancer factor 2B, Mef2b antibody specific to Mouse Mef2b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mef2b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mef2b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mef2b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Expanding Mouse Ventricular Cardiomyocytes Through GSK‐3 Inhibition
embryos. (B ) Mouse embryo at ∼E12.5. Dashed lines indicate where incisions should be made to yield the heart. (C ) Fetal mouse heart. Dashed lines indicate excision of atrial tissue. (D ) Pooling of ventricular tissue in 15‐ml tubes. Two to three tubes
of interest using a highfidelity enzyme such as Herculase II fusion polymerase ofKapa Hifi Hotstart polymerase. Typically an amplicon sizeof less than 1,000 bp is preferred as shorter amplicon givesmore specifi c amplifi cation products. (b) Visualize the PCR
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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