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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Antigen peptide transporter 2, Tap2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Antigen peptide transporter 2, Tap2 ELISA KIT
Product Name:Mouse Antigen peptide transporter 2, Tap2 ELISA KIT
Packing:96T
Catalog No.:ELI-13720m
Gene Name:Mouse Tap2
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Tap2
Alternative Name:Mouse Antigen peptide transporter 2, Tap2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Antigen peptide transporter 2, Tap2 ELISA KIT allows for the in vitro quantitative determination of Mouse Tap2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Antigen peptide transporter 2, Tap2 ELISA KIT has been pre-coated with an Mouse Antigen peptide transporter 2, Tap2 antibody specific to Mouse Tap2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tap2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tap2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tap2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Peptide Inhibition ELISA Protocol
Dilute specific and non-specific peptides to 200 mg/ml in PBT. In a microtiter plate, mix equal volumes of the peptide dilutions and the MAb supe. Add an equal volume of PBT and MAb as the control. Incubate 1 hour at 37
Peptide Inhibition ELISA Protocol
Peptide Inhibition ELISA Protocol Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
Antigen Design Sera Purification Tech Sheet
will briefly explore peptide selection and design, coupling strategy, and carrier proteins which are important factors in anti-peptide antisera generation. Serum purification will also be discussed. For more complete coverage of antigen design, please refer
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