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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Transcription factor jun-D, Jund
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Transcription factor jun- D, Jund ELISA KIT
Product Name:Mouse Transcription factor jun- D, Jund ELISA KIT
Packing:96T
Catalog No.:ELI-12642m
Gene Name:Mouse Jund
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Jund
Alternative Name:Mouse Transcription factor jun-D, Jund
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Transcription factor jun- D, Jund ELISA KIT allows for the in vitro quantitative determination of Mouse Jund concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Transcription factor jun- D, Jund ELISA KIT has been pre-coated with an Mouse Transcription factor jun-D, Jund antibody specific to Mouse Jund .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Jund and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Jund , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Jund in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验domain. This region is deleted in v-Jun. v-Jun is not phosphorylated but is transcriptionally active. Thus, binding of inactive SAPK to Jun may represent a mechanism for inhibiting the function of the transcription factor. Once activated, the SAPK
stability of p53, c-Myc, and c-Jun transcription factors. Especially, the purpose is to highlight the role of protein phosphorylation in the regulation of stability of these transcription factors. We also present examples where phosphorylation
Proteomic Methodologies to Study Transcription Factor Function
have been developed to improve both transcription factor and promoter characterization, some of which are discussed here. Trapping methodology applicable to both individual transcription factors and intact transcription complexes are described, as well as 2D gel
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