Hypoxanthine-guanine phosphori
bosyltransferase, Hprt1 ELI/ Mouse Hypoxanthine-guanine phosphoribosyltransferase, Hprt1 ELI- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-13082m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Hypoxanthine-guanine phosphoribosyltransferase, Hprt1 ELI
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Hypoxanthine- guanine phosphoribosyltransferase, HPRT1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
HPRT1,HGPRT; HPRT; HGPRTase; hypoxanthine-guanine phosphoribosyltransferase 1; hypoxanthine phosphoribosyltransferase 1, Hypoxanthine-guanine phosphoribosyltransferase
Search name
Mouse HPRT1 ELISA KIT ,Mouse HGPRT ELISA KIT ,Mouse HPRT ELISA KIT ,Mouse HGPRTase ELISA KIT ,Mouse hypoxanthine-guanine phosphoribosyltransferase 1 ELISA KIT ,Mouse hypoxanthine phosphoribosyltransferase 1 ELISA KIT ,Mouse Hypoxanthine-guanine phosphoribosyltransferase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse hypoxanthine-guanine phosphoribosyltransferase,HGPRT concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HGPRT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HGPRT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HGPRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HGPRT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes
-throughput method for identifying mouse spleen lymphocytes having mutations in the endogenous X-linked hypoxanthine-guanine phosphoribosyltransferase (Hprt ) gene and the endogenous autosomal thymidine kinase (Tk ) gene. The selective expansion of mutant
Developing Genetically Engineered Mouse Models to Study Tumor Suppression
Costantini, F. and Lacy, E. 1981. Introduction of a rabbit beta‐globin gene into the mouse germ line. Nature 294:92‐94. Cui, X., Ji, D., Fisher, D.A., Wu, Y
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
Homo sapiens matrix metalloproteinase-like 1 (MMPL1), mRNA 695 NM_001950 Homo sapiens E2F transcription factor 4, p107/p130-binding (E2F4), mRNA 956 NM_003815 Homo sapiens a disintegrin and metalloproteinase domain 15 (metargidin) (ADAM15), mRNA
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