- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-12086m
- 2026年01月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Akr1c18
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Akr1c18 ELISA KIT
Product Name:Mouse Akr1c18 ELISA KIT
Packing:96T
Catalog No.:ELI-12086m
Gene Name:Mouse Akr1c18
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Akr1c18
Alternative Name:Mouse Akr1c18
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Akr1c18 ELISA KIT allows for the in vitro quantitative determination of Mouse Akr1c18 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Akr1c18 ELISA KIT has been pre-coated with an Mouse Akr1c18 antibody specific to Mouse Akr1c18 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Akr1c18 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Akr1c18 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Akr1c18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
2基因有关，例如， C3H系与 AKR系二者都为 H－ 2k ，但其它基因则不同，例如 T细胞特异抗原的 Thy 1抗原，在 C3H为 Thy 1． 2，而 AKR为 Thy 1． 1，然而只将 AKR的 Thy 1． 1用 C3H的 Thy 1． 2置换成的 AKR-Thy 1． 2与原来的 AKR系对 Thy 1抗原之外的基因来说是同族系。关于形成同族鼠，以 Thy 1抗原为例，用 C3H与 AKR交配，以后以 Thy 1． 2抗原之有无为指标，与 AKR回交（ 10世代以上），这样在 Thy
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