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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白CDKN2AIP N-terminal-like protein, Cdkn2aipnl含量
- 适应物种:
小鼠
- 标记物:
CDKN2AIP N-terminal-like protein, Cdkn2aipnl
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse CDKN2AIP N- terminal- like protein, Cdkn2aipnl ELISA KIT
Product Name:Mouse CDKN2AIP N- terminal- like protein, Cdkn2aipnl ELISA KIT
Packing:96T
Catalog No.:ELI-11558m
Gene Name:Mouse Cdkn2aipnl
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Cdkn2aipnl
Alternative Name:Mouse CDKN2AIP N-terminal-like protein, Cdkn2aipnl
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse CDKN2AIP N- terminal- like protein, Cdkn2aipnl ELISA KIT allows for the in vitro quantitative determination of Mouse Cdkn2aipnl concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse CDKN2AIP N- terminal- like protein, Cdkn2aipnl ELISA KIT has been pre-coated with an Mouse CDKN2AIP N-terminal-like protein, Cdkn2aipnl antibody specific to Mouse Cdkn2aipnl .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cdkn2aipnl and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cdkn2aipnl , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cdkn2aipnl in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
. Protoc. Immunol. 21:A.3B.1‐A.3B.2. Wang, J., Rao, S., Chu, J., Shen, X., Levasseur, D.N., Theunissen, T.W., and Orkin, S.H. 2006. A protein interaction network for pluripotency
Performing Bronchoalveolar Lavage in the Mouse
lavage. Vet. Clin. Small Anim. 33:69‐88. Blé, F.‐X, Cannet, C., Zurbruegg, S., Karmouty Quintana, H., Frossard, N., Trifilieff, A., and Beckmann, N. 2008. Allergen‐induced lung
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