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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Complement C1r subcomponent-like protein, C1rl含量
- 适应物种:
小鼠
- 标记物:
Complement C1r subcomponent-like protein, C1rl
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Complement C1r subcomponent- like protein, C1rl ELISA KIT
Product Name:Mouse Complement C1r subcomponent- like protein, C1rl ELISA KIT
Packing:96T
Catalog No.:ELI-11556m
Gene Name:Mouse C1rl
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse C1rl
Alternative Name:Mouse Complement C1r subcomponent-like protein, C1rl
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Complement C1r subcomponent- like protein, C1rl ELISA KIT allows for the in vitro quantitative determination of Mouse C1rl concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Complement C1r subcomponent- like protein, C1rl ELISA KIT has been pre-coated with an Mouse Complement C1r subcomponent-like protein, C1rl antibody specific to Mouse C1rl .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse C1rl and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse C1rl , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse C1rl in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
Platinum Taq PCR x DNA Polymerase (Invitrogen; or equivalent commercial high‐fidelity Taq polymerase kit), including: 10× PCR buffer
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Monoclonal Antibody Production
a pre-bleed on them to be used as an ELISA control for monitoring the titer and screening of the hybridomas: Bleeding Mice Place the mouse in a mouse restrainer. Sterilize the tail with 70% ethanol. With a razor blade, nip
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