- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-11406m
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白CD180 antigen, Cd180含量
- 适应物种：
小鼠
- 标记物：
CD180 antigen, Cd180
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse CD180 antigen, Cd180 ELISA KIT
Product Name:Mouse CD180 antigen, Cd180 ELISA KIT
Packing:96T
Catalog No.:ELI-11406m
Gene Name:Mouse Cd180
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Cd180
Alternative Name:Mouse CD180 antigen, Cd180
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse CD180 antigen, Cd180 ELISA KIT allows for the in vitro quantitative determination of Mouse Cd180 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse CD180 antigen, Cd180 ELISA KIT has been pre-coated with an Mouse CD180 antigen, Cd180 antibody specific to Mouse Cd180 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cd180 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cd180 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cd180 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验thousands of colored products. Thus, in ELISA, antigens are detectable when present in only nanogram and picogram quantities. One form of the ELISA is carried out in a microtiter plate which is used as the solid phase for adsorption of antigen (Figure
Antigen Design Sera Purification Tech Sheet
will briefly explore peptide selection and design, coupling strategy, and carrier proteins which are important factors in anti-peptide antisera generation. Serum purification will also be discussed. For more complete coverage of antigen design, please refer
as antigen. 2. In a 96-well ELISA plate (Nunc MaxiSorp is best), add 100 ng of antigen in 50 µL in each well you will be using for your test, as well as four control wells. The perimeter wells on the plate are generally not used, as they tend to give poor
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