Actin-related protein 10, Actr
10 ELISA KIT/ Mouse Actin-related protein 10, Actr10 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-11356m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Actin-related protein 10, Actr10含量
- 适应物种：
小鼠
- 标记物：
Actin-related protein 10, Actr10
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Actin- related protein 10, Actr10 ELISA KIT
Product Name:Mouse Actin- related protein 10, Actr10 ELISA KIT
Packing:96T
Catalog No.:ELI-11356m
Gene Name:Mouse Actr10
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Actr10
Alternative Name:Mouse Actin-related protein 10, Actr10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Actin- related protein 10, Actr10 ELISA KIT allows for the in vitro quantitative determination of Mouse Actr10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Actin- related protein 10, Actr10 ELISA KIT has been pre-coated with an Mouse Actin-related protein 10, Actr10 antibody specific to Mouse Actr10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Actr10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Actr10 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Actr10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验10. (take muscle F-actin; not supernatant) homogenize in 300 ml buffer G at +4 oC; dialyze suspension against buffer G for 48 h; centrifuge at 100,000 x g for 2 h 11. (take muscle G-actin; not pellet) chromatograph on sephacryl S-300 column
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Combine actin + GST-fusion protein and incubate for 1 hr. x 4¡C. Add 20ul 50% glutathione agarose equilibrated into binding buffer. Incubate 15 min x 4¡C with frequent mixing. Wash beads 4 times with binding buffer, spin down 4-8000 rpm
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