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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白T-cell surface glycoprotein CD3 zeta, Cd247含量
- 适应物种:
小鼠
- 标记物:
T-cell surface glycoprotein CD3 zeta, Cd247
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse T- cell surface glycoprotein CD3 zeta, CD247 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CD247,CD247 molecule; CD247 antigen, zeta subunit; CD3-ZETA; CD3H; CD3Q; CD3Z; T3Z; TCRZ; CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; TCR zeta chain
Search name
Mouse CD247 ELISA KIT ,Mouse CD247 molecule ELISA KIT ,Mouse CD247 antigen zeta subunit ELISA KIT ,Mouse CD247 antigen ELISA KIT ,Mouse CD3-ZETA ELISA KIT ,Mouse CD3H ELISA KIT ,Mouse CD3Q ELISA KIT ,Mouse CD3Z ELISA KIT ,Mouse T3Z ELISA KIT ,Mouse TCRZ ELISA KIT ,Mouse CD3Z antigen ELISA KIT ,Mouse zeta polypeptide (TiT3 complex) ELISA KIT ,Mouse CD3zeta chain ELISA KIT ,Mouse T-cell antigen receptor complex ELISA KIT ,Mouse zeta subunit of CD3 ELISA KIT ,Mouse T-cell receptor T3 zeta chain ELISA KIT ,Mouse T-cell surface glycoprotein CD3 zeta chain ELISA KIT ,Mouse TCR zeta chain ELISA KIT ,Mouse T-cell surface glycoprotein CD3 zeta ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse T- cell surface glycoprotein CD3 zeta, CD247 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse T- cell surface glycoprotein CD3 zeta, CD247, During the reaction, Mouse T- cell surface glycoprotein CD3 zeta, CD247 in the sample or standard competes with a fixed amount of biotin-labeled Mouse T- cell surface glycoprotein CD3 zeta, CD247 for sites on a pre-coated Monoclonal antibody specific to Mouse T- cell surface glycoprotein CD3 zeta, CD247. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse T- cell surface glycoprotein CD3 zeta, CD247 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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