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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Chloride channel protein 1, Clcn1含量
- 适应物种:
小鼠
- 标记物:
Chloride channel protein 1, Clcn1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Chloride channel protein 1, Clcn1 ELISA KIT
Product Name:Mouse Chloride channel protein 1, Clcn1 ELISA KIT
Packing:96T
Catalog No.:ELI-10180m
Gene Name:Mouse Clcn1
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Clcn1
Alternative Name:Mouse Chloride channel protein 1, Clcn1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Chloride channel protein 1, Clcn1 ELISA KIT allows for the in vitro quantitative determination of Mouse Clcn1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Chloride channel protein 1, Clcn1 ELISA KIT has been pre-coated with an Mouse Chloride channel protein 1, Clcn1 antibody specific to Mouse Clcn1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Clcn1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Clcn1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Clcn1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Measurement of Chloride Movement in Neuronal Preparations
. Functional diversity of GABA‐activated chloride currents in Purkinje versus granule neurons in rat cerebellar slices. Neuron 12:117‐126. Schwartz, R.D. and Yu, X. 1995. Optical
CFTR is the only member of the ABC (ATP-binding cassette) protein superfamily known to function as an ion channel. Most other ABC proteins are ATP-driven transporters, in which a cycle of ATP binding and hydrolysis, at intracellular
Probing CFTR Channel Structure and Function Using the Substituted-Cysteine-Accessibility Method
The cystic fibrosis transmembrane conductance regulator (CFTR) forms a chloride channel whose activation is regulated by phosphorylation and by ATP binding and hydrolysis (1 -4 ). The functional properties of the channel have been extensively
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