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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Uncharacterized protein CXorf65 homolog, Gm614含量
- 适应物种:
小鼠
- 标记物:
Uncharacterized protein CXorf65 homolog, Gm614
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Uncharacterized protein CXorf65 homolog, Gm614 ELISA KIT
Product Name:Mouse Uncharacterized protein CXorf65 homolog, Gm614 ELISA KIT
Packing:96T
Catalog No.:ELI-09929m
Gene Name:Mouse Gm614
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Gm614
Alternative Name:Mouse Uncharacterized protein CXorf65 homolog, Gm614
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Uncharacterized protein CXorf65 homolog, Gm614 ELISA KIT allows for the in vitro quantitative determination of Mouse Gm614 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Uncharacterized protein CXorf65 homolog, Gm614 ELISA KIT has been pre-coated with an Mouse Uncharacterized protein CXorf65 homolog, Gm614 antibody specific to Mouse Gm614 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gm614 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gm614 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gm614 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
), nuclear gene encoding mitochondrial protein, mRNA 2307 NM_001654 Homo sapiens v-raf murine sarcoma 3611 viral oncogene homolog 1 (ARAF1), mRNA 846 NM_002967 Homo sapiens scaffold attachment factor B (SAFB ), mRNA 545 NM_001183 Homo sapiens ATPase
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
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