Chloride channel protein ClC-K
a, Clcnka ELISA KIT/ Mouse Chloride channel protein ClC-Ka, Clcnka ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-10599m
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Chloride channel protein ClC-Ka, Clcnka含量
- 适应物种：
小鼠
- 标记物：
Chloride channel protein ClC-Ka, Clcnka
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Chloride channel protein ClC- Ka, Clcnka ELISA KIT
Product Name:Mouse Chloride channel protein ClC- Ka, Clcnka ELISA KIT
Packing:96T
Catalog No.:ELI-10599m
Gene Name:Mouse Clcnka
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Clcnka
Alternative Name:Mouse Chloride channel protein ClC-Ka, Clcnka
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Chloride channel protein ClC- Ka, Clcnka ELISA KIT allows for the in vitro quantitative determination of Mouse Clcnka concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Chloride channel protein ClC- Ka, Clcnka ELISA KIT has been pre-coated with an Mouse Chloride channel protein ClC-Ka, Clcnka antibody specific to Mouse Clcnka .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Clcnka and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Clcnka , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Clcnka in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Measurement of Chloride Movement in Neuronal Preparations
. Cala, P.M. 1990. Principles of cell volume regulation: Ion flux pathways and the roles of anions. In Chloride Channels and Carriers in Nerve, Muscle, and Glial Cells (F.J. Alvarez‐Leefmans and J.M. Russell eds
to a goat antimouse IgG, which catalyzes the substrate p-nitrophenyl phosphate, to form a colored product. Excess reagents are removed by washing, and enzyme activity can be measured with an ELISA plate reader at 415nm. The amount of colored product
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
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