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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Centromere protein N, Cenpn含量
- 适应物种:
小鼠
- 标记物:
Centromere protein N, Cenpn
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Centromere protein N, Cenpn ELISA KIT
Product Name:Mouse Centromere protein N, Cenpn ELISA KIT
Packing:96T
Catalog No.:ELI-10545m
Gene Name:Mouse Cenpn
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Cenpn
Alternative Name:Mouse Centromere protein N, Cenpn
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Centromere protein N, Cenpn ELISA KIT allows for the in vitro quantitative determination of Mouse Cenpn concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Centromere protein N, Cenpn ELISA KIT has been pre-coated with an Mouse Centromere protein N, Cenpn antibody specific to Mouse Cenpn .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cenpn and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cenpn , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cenpn in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
by purification of selected phage by ELISA. Alternatively, there is a bead?based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic
. Protoc. Immunol. 21:A.3B.1‐A.3B.2. Wang, J., Rao, S., Chu, J., Shen, X., Levasseur, D.N., Theunissen, T.W., and Orkin, S.H. 2006. A protein interaction network for pluripotency
Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example
in incomplete Freund’s adjuvant, the respective antisera were collected and assayed by enzyme-linked immunosorbent assay (ELISA) and purified by affinity chromatography on peptides conjugated to SulfoLink Coupling Gel (Pierce). Mouse brain proteins were prepared
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