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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Collagen alpha-1(XXIV) chain, Col24a1含量
- 适应物种:
小鼠
- 标记物:
Collagen alpha-1(XXIV) chain, Col24a1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Collagen alpha- 1(XXIV) chain, Col24a1 ELISA KIT
Product Name:Mouse Collagen alpha- 1(XXIV) chain, Col24a1 ELISA KIT
Packing:96T
Catalog No.:ELI-10477m
Gene Name:Mouse Col24a1
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Col24a1
Alternative Name:Mouse Collagen alpha-1(XXIV) chain, Col24a1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Collagen alpha- 1(XXIV) chain, Col24a1 ELISA KIT allows for the in vitro quantitative determination of Mouse Col24a1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Collagen alpha- 1(XXIV) chain, Col24a1 ELISA KIT has been pre-coated with an Mouse Collagen alpha-1(XXIV) chain, Col24a1 antibody specific to Mouse Col24a1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Col24a1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Col24a1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Col24a1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验The enzyme-linked immunosorbent assay (ELISA) is a widely used technique for detecting antibodies (Abs) and is employed in clinical laboratories to identify Abs against various self-antigens–autoAb development and quantitation. This method
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