Lanosterol 14-alpha demethylas
e, Cyp51a1 ELISA KIT/ Mouse Lanosterol 14-alpha demethylase, Cyp51a1 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-10414m
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Lanosterol 14-alpha demethylase, Cyp51a1含量
- 适应物种：
小鼠
- 标记物：
Lanosterol 14-alpha demethylase, Cyp51a1
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Lanosterol 14- alpha demethylase, Cyp51a1 ELISA KIT
Product Name:Mouse Lanosterol 14- alpha demethylase, Cyp51a1 ELISA KIT
Packing:96T
Catalog No.:ELI-10414m
Gene Name:Mouse Cyp51a1
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Cyp51a1
Alternative Name:Mouse Lanosterol 14-alpha demethylase, Cyp51a1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Lanosterol 14- alpha demethylase, Cyp51a1 ELISA KIT allows for the in vitro quantitative determination of Mouse Cyp51a1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Lanosterol 14- alpha demethylase, Cyp51a1 ELISA KIT has been pre-coated with an Mouse Lanosterol 14-alpha demethylase, Cyp51a1 antibody specific to Mouse Cyp51a1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cyp51a1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cyp51a1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cyp51a1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Azole antifungal drugs are used widely to treat people with oral fungal infections. Unfortunately, fungi can develop resistance to these drugs. This resistance can be due to the overexpression or mutation of cytochrome P450 14α-lanosterol
Purification and Quantification of Saccharomyces cerevisiae Cytochrome P450
are membrane-bound, in most cases located in the endoplasmic reticulum. Higher eukaryotes often have complex systems containing many cyt.P450s. However, vegetatively growing Saccharomyces cerevisiae contains only one major cyt.P450, the lanosterol 14α
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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