- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-10412m
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Vitamin D 25-hydroxylase, Cyp2r1含量
- 适应物种：
小鼠
- 标记物：
Vitamin D 25-hydroxylase, Cyp2r1
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Vitamin D 25- hydroxylase, CYP2R1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Cyp2r1,cytochrome P450 2R1; cytochrome P450, family 2, R1; vitamin D 25-hydroxylase; cytochrome P450, family 2, subfamily R, polypeptide 1
Search name
Mouse Cyp2r1 ELISA KIT ,Mouse cytochrome P450 2R1 ELISA KIT ,Mouse cytochrome P450 family 2 R1 ELISA KIT ,Mouse vitamin D 25-hydroxylase ELISA KIT ,Mouse cytochrome P450 family 2 subfamily R polypeptide 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Vitamin D 25- hydroxylase, CYP2R1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D 25- hydroxylase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Vitamin D 25- hydroxylase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Vitamin D 25- hydroxylase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Vitamin D 25- hydroxylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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