Mouse Vitamin D 25-hydroxylase

Mouse Vitamin D 25- hydroxylase, CYP2R1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Cyp2r1,cytochrome P450 2R1; cytochrome P450, family 2, R1; vitamin D 25-hydroxylase; cytochrome P450, family 2, subfamily R, polypeptide 1

Search name

Mouse Cyp2r1 ELISA KIT ,Mouse cytochrome P450 2R1 ELISA KIT ,Mouse cytochrome P450 family 2 R1 ELISA KIT ,Mouse vitamin D 25-hydroxylase ELISA KIT ,Mouse cytochrome P450 family 2 subfamily R polypeptide 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Vitamin D 25- hydroxylase, CYP2R1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D 25- hydroxylase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Vitamin D 25- hydroxylase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Vitamin D 25- hydroxylase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Vitamin D 25- hydroxylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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