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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Cbp含量
- 适应物种:
小鼠
- 标记物:
Cbp
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cbp/p300- interacting transactivator 1, Cited1 ELISA KIT
Product Name:Mouse Cbp/p300- interacting transactivator 1, Cited1 ELISA KIT
Packing:96T
Catalog No.:ELI-10208m
Gene Name:Mouse Cited1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Cited1
Alternative Name:Mouse Cbp/p300-interacting transactivator 1, Cited1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cbp/p300- interacting transactivator 1, Cited1 ELISA KIT allows for the in vitro quantitative determination of Mouse Cited1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cbp/p300- interacting transactivator 1, Cited1 ELISA KIT has been pre-coated with an Mouse Cbp/p300-interacting transactivator 1, Cited1 antibody specific to Mouse Cited1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cited1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cited1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cited1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
质乙酰化修饰研究进展)目前,对于 p53 的乙酰化修饰已经研究得较为清楚,在 p300/CBP 的催化下,p53 的 C 端 DNA 结合调控区域上发生多个赖氨酸位点的乙酰化修饰,从而激活 p53 上特异 DNA 结合区域的活化。还比如 AP-1,ATF-5,BMAL1,CBP,Cytokeratin,E2F-4,EF-1,HMG-1,Hsp90,Hsp70,ku-70,stat3,Ub,NF-E4,NF-Kb-p65 P73,Nrf2,P300,PTEN,Ref-1 等,修饰后的蛋白质可以对细胞
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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