Flotillin-1, Flot1 ELISA KIT/

Mouse Flotillin- 1, Flot1 ELISA KIT

Product Name:Mouse Flotillin- 1, Flot1 ELISA KIT
Packing:96T

Catalog No.:ELI-09848m

Gene Name:Flot1

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Mouse Flotillin- 1

Alternative Name:Flot1,Mouse Flotillin- 1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Flotillin- 1, Flot1 ELISA KIT allows for the in vitro quantitative determination ofMouse Flotillin- 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Flotillin- 1, Flot1 ELISA KIT has been pre-coated with an Flot1 antibody specific to Mouse Flotillin- 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Flotillin- 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Flotillin- 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Flotillin- 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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