F-box only protein 30, Fbxo30
ELISA KIT/ Mouse F-box only protein 30, Fbxo30 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-09506m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白F-box only protein 30, Fbxo30含量
- 适应物种：
小鼠
- 标记物：
F-box only protein 30, Fbxo30
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse F- box only protein 30, Fbxo30 ELISA KIT
Product Name:Mouse F- box only protein 30, Fbxo30 ELISA KIT
Packing:96T
Catalog No.:ELI-09506m
Gene Name:Mouse Fbxo30
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Fbxo30
Alternative Name:Mouse F-box only protein 30, Fbxo30
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse F- box only protein 30, Fbxo30 ELISA KIT allows for the in vitro quantitative determination of Mouse Fbxo30 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse F- box only protein 30, Fbxo30 ELISA KIT has been pre-coated with an Mouse F-box only protein 30, Fbxo30 antibody specific to Mouse Fbxo30 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Fbxo30 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Fbxo30 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Fbxo30 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Typical modification sites: RGG box or RXR sequence motifs R-arginine, G-glycine, X-any aminoacid. Enzymes catalysing protein arginine methylation: PRMT1 from rat and the human homologue HRMT1L2, human PRMT2 (HRMT1L1), rat and human PRMT
Alternate Protocol 1: RNA Preparation from Pure RPE Cells Basic Protocol 4: Protein Preparation from Eye Tissues Basic Protocol 5: Fixation, Inclusion, and Cryosection of the Mouse Eye
The sandwich ELISA measures the amount of antigen between two layers of antibodies. The antigens to be measured must contain at least two antigenic sites, capable of binding to antibody, since at least two antibodies act in the sandwich
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