Mouse 25-hydroxycholesterol 7-alpha-hydroxylase, Cyp7b1 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Mouse Cyp7b1 ELISA KIT ,Mouse CBAS3 ELISA KIT ,Mouse CP7B ELISA KIT ,Mouse SPG5A ELISA KIT ,Mouse cytochrome P450 7B1 ELISA KIT ,Mouse cytochrome P450 family 7 subfamily B polypeptide 1 ELISA KIT ,Mouse cytochrome P450 subfamily VIIB (oxysterol 7 alpha-hydroxylase) polypeptide 1 ELISA KIT ,Mouse oxysterol 7-alpha-hydroxylase ELISA KIT ,Mouse cytochrome P450 family 7 subfamily B member 1 ELISA KIT ,Mouse 25-hydroxycholesterol 7-alpha-hydroxylase ELISA KIT ,Mouse Oxysterol 7-alpha-hydroxylase ELISA KIT ,Mouse Oxysterol 7-alpha-hydroxylase ELISA KIT ,Mouse Cytochrome P450 7B1 ELISA KIT ,Mouse Hippocampal transcript 1 protein ELISA KIT ,Mouse HCT-1 ELISA KIT
Intended use
Mouse Cyp7b1 ELISA kit allows for the in vitro quantitative determination of Mouse Cyp7b1 concentmouseions in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Mouse Cyp7b1 ELISA kit has been pre-coated with an antibody specific to Cyp7b1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody prepamouseion specific for Cyp7b1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substmousee solution is added to each well. Only those wells that contain Cyp7b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substmousee reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentmouseion of Cyp7b1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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