- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-09242m
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白DNA polymerase beta, Polb含量
- 适应物种：
小鼠
- 标记物：
DNA polymerase beta, Polb
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse DNA polymerase beta, Polb ELISA KIT
Product Name:Mouse DNA polymerase beta, Polb ELISA KIT
Packing:96T
Catalog No.:ELI-09242m
Gene Name:Mouse Polb
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Polb
Alternative Name:Mouse DNA polymerase beta, Polb
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse DNA polymerase beta, Polb ELISA KIT allows for the in vitro quantitative determination of Mouse Polb concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse DNA polymerase beta, Polb ELISA KIT has been pre-coated with an Mouse DNA polymerase beta, Polb antibody specific to Mouse Polb .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Polb and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Polb , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Polb in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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immunosorbent assay (PCR-ELISA). The antibody was first immobilized on antigen-coated microplates; in a second step, a commercially available DNA-labeled species-specific antibody was added; and finally the deoxyribonucleic marker was amplified in a PCR step
相关专题 重组DNA技术工具酶 定义 DNA聚合酶 (DNA polymerase)是细胞复制DNA的重要作用酶。 DNA聚合酶 , 以DNA为复制模板，从将DNA由5'端点开始复制到3'端的酶。 DNA聚合酶 的主要活性是催化DNA的合成(在具备模板、引物、dNTP等的情况下)及其相辅的活性。 真核细胞有5种DNA聚合酶，分别为DNA聚合酶α(定位于胞核
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