- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-09214m
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白DNA ligase 3, Lig3含量
- 适应物种：
小鼠
- 标记物：
DNA ligase 3, Lig3
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse DNA ligase 3, Lig3 ELISA KIT
Product Name:Mouse DNA ligase 3, Lig3 ELISA KIT
Packing:96T
Catalog No.:ELI-09214m
Gene Name:Mouse Lig3
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Lig3
Alternative Name:Mouse DNA ligase 3, Lig3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse DNA ligase 3, Lig3 ELISA KIT allows for the in vitro quantitative determination of Mouse Lig3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse DNA ligase 3, Lig3 ELISA KIT has been pre-coated with an Mouse DNA ligase 3, Lig3 antibody specific to Mouse Lig3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Lig3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Lig3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Lig3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Production of Monoclonal Antibody by DNA Immunization with Electroporation
construct was injected intramuscularly with in vivo electroporation into mice, and the specific antibody production in mice was analyzed by enzyme-linked immunosorbent assay (ELISA). Hybridomas were generated by fusing mouse splenocytes with myeloma cells
A Comprehensive Guide to Sleeping Beauty–Based Somatic Transposon Mutagenesis in the Mouse
) 5 M NaCl T4 DNA ligase and 10× buffer (New England Biolabs) Bam HI (New England Biolabs
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
to the concentration of 100 mM. Store at -20°C. 6. T4 DNA ligase and ligation reaction buffer (Promega). Storeat ?20°C. 7. PCR grade water or Millipore purified water. 8. Biolase Taq DNA polymerase (Bioline USA Inc.). Store at?20°C. 9. 10× PCR
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