- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-08977m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Beta-defensin 20, Defb20含量
- 适应物种：
小鼠
- 标记物：
Beta-defensin 20, Defb20
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Beta- defensin 20, Defb20 ELISA KIT
Product Name:Mouse Beta- defensin 20, Defb20 ELISA KIT
Packing:96T
Catalog No.:ELI-08977m
Gene Name:Mouse Defb20
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Defb20
Alternative Name:Mouse Beta-defensin 20, Defb20
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Beta- defensin 20, Defb20 ELISA KIT allows for the in vitro quantitative determination of Mouse Defb20 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Beta- defensin 20, Defb20 ELISA KIT has been pre-coated with an Mouse Beta-defensin 20, Defb20 antibody specific to Mouse Defb20 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Defb20 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Defb20 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Defb20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验人b-防御素(b-Defensin)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 b -防御素 ( b -Defensin)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 b -Defensin 单抗包被于酶标板上，标准品和样品中的 b -Defensin与单抗
真爱满行囊 我最近在体外细胞做的关于gsk3-beta功能问题，发现药物处理以后，gsk-3-beta的总表达量下调，如果是这样的话 1、我是否能够得出gsk-3-beta的活性下调的结论？ 2、是否还有必要做非活性形式的表达量？我的理解是，基础状态下，gsk-3beta维持的是低活性状态，如果总量都下调了，那应该能够得出活性降低的结论了吧？ 3、另外，在这种情况下我是否有必要去做gsk-3-beta的216位点的活性形式的表达
FA Extraction of [insoluble] Amyloid beta from Mouse Brain
each sample individually for one minute on ice. Immerse the tip of the probe in the sample, then move tube up and down while sonicating. 3. Spin 400 μl at 135,000 x g for 1 hr at 4°C (50,000 rpm in a TLA 100.3 rotor). 4. Dilute 210 μl
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