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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Keratinocyte differentiation-associated protein, Krtdap E含量
- 适应物种:
小鼠
- 标记物:
Keratinocyte differentiation-associated protein, Krtdap E
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Keratinocyte differentiation- associated protein, KRTDAP ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
KRTDAP,KDAP; UNQ467; KIPV467; keratinocyte differentiation-associated protein
Search name
Mouse KRTDAP ELISA KIT ,Mouse KDAP ELISA KIT ,Mouse UNQ467 ELISA KIT ,Mouse KIPV467 ELISA KIT ,Mouse keratinocyte differentiation-associated protein ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Keratinocyte differentiation- associated protein, KRTDAP concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to KRTDAP . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for KRTDAP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain KRTDAP , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of KRTDAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized Product
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文献和实验Generation of Functional Multipotent Keratinocytes from Mouse Induced Pluripotent Stem Cells
and efficiency of keratinocyte derivation depending on the mouse genetic background used in the study. Both protocols employ applications of retinoic acid and bone-morphogenetic protein-4 and growth on collagen type IV-coated dishes to induce iPSC differentiation
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
and maintained in the form of neurospheres and, upon removal of these growth factors, can efficiently generate the three major CNS cell types. Advances in mouse genetic manipulation, as well as the development of more powerful analytical technologies (e
Phenotypic Analysis of BAT versus WAT Differentiation
that overcome these limitations. Curr. Protoc. Mouse Biol . 3:205?216 © 2013 by John Wiley & Sons, Inc. Keywords: white adipose tissue; brown adipose tissue; adipogenesis; adipocytes; FACS; cryosectioning
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