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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Lipid phosphate phosphohydrolase 3, Ppap2b含量
- 适应物种:
小鼠
- 标记物:
Lipid phosphate phosphohydrolase 3, Ppap2b
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Lipid phosphate phosphohydrolase 3, Ppap2b ELISA KIT
Product Name:Mouse Lipid phosphate phosphohydrolase 3, Ppap2b ELISA KIT
Packing:96T
Catalog No.:ELI-08603m
Gene Name:Mouse Ppap2b
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Ppap2b
Alternative Name:Mouse Lipid phosphate phosphohydrolase 3, Ppap2b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Lipid phosphate phosphohydrolase 3, Ppap2b ELISA KIT allows for the in vitro quantitative determination of Mouse Ppap2b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Lipid phosphate phosphohydrolase 3, Ppap2b ELISA KIT has been pre-coated with an Mouse Lipid phosphate phosphohydrolase 3, Ppap2b antibody specific to Mouse Ppap2b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ppap2b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ppap2b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ppap2b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
membrane protein or 0.1 to 0.2 mM lipid phosphate TCA/TBA/HCl solution (see recipe ) Boiling
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Characterization of Whole Body Cholesterol Fluxes in the Mouse
Wang, D.Q.H. and Carey, M.C. 2003. Measurement of intestinal cholesterol absorption by plasma and fecal dual‐isotope ratio, mass balance, and lymph fistula methods in the mouse. J. Lipid Res. 44:1042–1059
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