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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Kallikrein 1-related peptidase b22, Klk1b22含量
- 适应物种:
小鼠
- 标记物:
Kallikrein 1-related peptidase b22, Klk1b22
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Kallikrein 1- related peptidase b22, Klk1b22 ELISA KIT
Product Name:Mouse Kallikrein 1- related peptidase b22, Klk1b22 ELISA KIT
Packing:96T
Catalog No.:ELI-08058m
Gene Name:Klk1b22
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Mouse Kallikrein 1- related peptidase b22
Alternative Name:Klk1b22,Mouse Kallikrein 1- related peptidase b22
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Kallikrein 1- related peptidase b22, Klk1b22 ELISA KIT allows for the in vitro quantitative determination ofMouse Kallikrein 1- related peptidase b22 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Kallikrein 1- related peptidase b22, Klk1b22 ELISA KIT has been pre-coated with an Klk1b22 antibody specific to Mouse Kallikrein 1- related peptidase b22 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Kallikrein 1- related peptidase b22 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Kallikrein 1- related peptidase b22, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Kallikrein 1- related peptidase b22 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Analysis of Oxidative Modification of Proteins
X‐ray film Additional reagents and equipment for SDS‐PAGE (unit 6.1 ) and staining gels with Coomassie blue R‐250 (unit
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