Mouse Diacylglycerol kinase alpha, Dgka ELISA KIT
Product Name:Mouse Diacylglycerol kinase alpha, Dgka ELISA KIT
Packing:96T
Catalog No.:ELI-07743m
Gene Name:Mouse Dgka
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Dgka
Alternative Name:Mouse Diacylglycerol kinase alpha, Dgka
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Diacylglycerol kinase alpha, Dgka ELISA KIT allows for the in vitro quantitative determination of Mouse Dgka concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Diacylglycerol kinase alpha, Dgka ELISA KIT has been pre-coated with an Mouse Diacylglycerol kinase alpha, Dgka antibody specific to Mouse Dgka .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dgka and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dgka , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dgka in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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