- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-07692m
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Integrin alpha-5, Itga5含量
- 适应物种：
小鼠
- 标记物：
Integrin alpha-5, Itga5
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Integrin alpha- 5, Itga5 ELISA KIT
Product Name:Mouse Integrin alpha- 5, Itga5 ELISA KIT
Packing:96T
Catalog No.:ELI-07692m
Gene Name:Mouse Itga5
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Itga5
Alternative Name:Mouse Integrin alpha-5, Itga5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Integrin alpha- 5, Itga5 ELISA KIT allows for the in vitro quantitative determination of Mouse Itga5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Integrin alpha- 5, Itga5 ELISA KIT has been pre-coated with an Mouse Integrin alpha-5, Itga5 antibody specific to Mouse Itga5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Itga5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Itga5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Itga5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验marrow. The prime suspect: an adhesion molecule called alpha2 integrin. The molecule showed increased expression on memory cells, and its ligand is predominantly expressed in bone marrow tissue. Also, when the researchers blocked alpha2 integrin
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
(yellow when acid is added). 2) Coat plate with antigen stock solution (1ug/ml in PBS, 50ul/well) for at least 24hrs or over the weekend at 4°C or O/N at 37°C. 3) Remove antigen and wash 3x with 1x PBS. 4) Block with 1% BSA (100ul/well) in 1x PBS
(交流)ELISA (Enzyme-Linked ImmunoadSorbent Assay)
ELISA (Enzyme-Linked ImmunoadSorbent Assay)You will need the following solutions:PBS (Phosphate-Buffered Saline, Ca/Mg free) :-Solute 1x 10xNaCl 8g 80gKCl 0.2g 2gNa2PO4 (anhydrous) 1.15g 11.5gKH2PO4 (anhydrous) 0.2g 2gN.B.: Na2HPO4.2
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