NADPH oxidase 1, Nox1 ELISA KI

Mouse NADPH oxidase 1,NOX1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

NOX1,NOX-1,MOX-1,GP91-2; MOX1; NOH-1; NOH1; NADH/NADPH mitogenic oxidase subunit P65-MOX; NADPH oxidase homolog-1; mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating); mitogenic oxidase 1; NADPH oxidase 1

Search name

Mouse NOX1 ELISA KIT ,Mouse NOX-1 ELISA KIT ,Mouse MOX-1 ELISA KIT ,Mouse GP91-2 ELISA KIT ,Mouse MOX1 ELISA KIT ,Mouse NOH-1 ELISA KIT ,Mouse NOH1 ELISA KIT ,Mouse NADH/NADPH mitogenic oxidase subunit P65-MOX ELISA KIT ,Mouse NADPH oxidase homolog-1 ELISA KIT ,Mouse mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating) ELISA KIT ,Mouse mitogenic oxidase 1 ELISA KIT ,Mouse NADPH oxidase 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse nadph oxidase 1,NOX-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to NOX-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NOX-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NOX-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NOX-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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