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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Adipocyte enhancer-binding protein 1, Aebp1含量
- 适应物种:
小鼠
- 标记物:
Adipocyte enhancer-binding protein 1, Aebp1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Adipocyte enhancer- binding protein 1, Aebp1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Adipocyte enhancer-binding protein 1,AE-binding protein 1,Aortic carboxypeptidase-like protein, Aebp1,ACLP; adipocyte enhancer-binding protein 1; aortic carboxypeptidase-like protein; AE binding protein 1
Search name
Mouse Adipocyte enhancer-binding protein 1 ELISA KIT ,Mouse AE-binding protein 1 ELISA KIT ,Mouse Aortic carboxypeptidase-like protein ELISA KIT ,Mouse Aebp1 ELISA KIT ,Mouse ACLP ELISA KIT ,Mouse adipocyte enhancer-binding protein 1 ELISA KIT ,Mouse aortic carboxypeptidase-like protein ELISA KIT ,Mouse AE binding protein 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse adipocyte enhancer-binding protein 1,AE-binding protein 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to AE-binding protein 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AE-binding protein 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AE-binding protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AE-binding protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Adipose Tissue-Specific PPAR Gene Targeting
1 and 2 of PPARγ and creation of the aP2-Cre transgenic mice, which express Cre recombinase under the control of the promoter of adipocyte fatty acid binding protein (aP2). Crossing of the two mouse lines results in deletion of PPARγ gene
Comparative Protein Structure Modeling Using MODELLER
Figure 2.9.12 Typical errors in comparative modeling. (A ) Errors in side chain packing. The Trp 109 residue in the crystal structure of mouse cellular retinoic acid binding protein I (red) is compared
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