- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-07536m
- 2025年07月05日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Protein Wnt-10a, Wnt10a含量
- 适应物种：
小鼠
- 标记物：
Protein Wnt-10a, Wnt10a
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Protein Wnt- 10a, Wnt10a ELISA KIT
Product Name:Mouse Protein Wnt- 10a, Wnt10a ELISA KIT
Packing:96T
Catalog No.:ELI-07536m
Gene Name:Mouse Wnt10a
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Wnt10a
Alternative Name:Mouse Protein Wnt-10a, Wnt10a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Protein Wnt- 10a, Wnt10a ELISA KIT allows for the in vitro quantitative determination of Mouse Wnt10a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Protein Wnt- 10a, Wnt10a ELISA KIT has been pre-coated with an Mouse Protein Wnt-10a, Wnt10a antibody specific to Mouse Wnt10a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Wnt10a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Wnt10a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Wnt10a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
v468.chapter 1 经典 Wnt/b-Catenin 信号通路 （英文完整版）
, b-catenin , Groucho , Tcf , Target gene , Constitutive activation , Stem cell ,Colon cancer. 1. Introduction The Wnt pathway derives its name from the Drosophila (fruit-fly)W ingless gene and the mouse I NT -1 gene. The mouse Wnt1gene
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