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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Neurogenic locus notch homolog protein 2, Notch2含量
- 适应物种:
小鼠
- 标记物:
Neurogenic locus notch homolog protein 2, Notch2
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Neurogenic locus notch homolog protein 2,NOTCH2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NOTCH2,AGS2; HJCYS; hN2; Notch homolog 2; neurogenic locus notch homolog protein 2; notch 2,
Search name
Mouse NOTCH2 ELISA KIT ,Mouse AGS2 ELISA KIT ,Mouse HJCYS ELISA KIT ,Mouse hN2 ELISA KIT ,Mouse Notch homolog 2 ELISA KIT ,Mouse neurogenic locus notch homolog protein 2 ELISA KIT ,Mouse notch 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse neurogenic locus notch homolog protein 2,NOTCH2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NOTCH2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NOTCH2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NOTCH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NOTCH2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
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