Neurogenic locus notch homolog

Mouse Neurogenic locus notch homolog protein 2,NOTCH2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

NOTCH2,AGS2; HJCYS; hN2; Notch homolog 2; neurogenic locus notch homolog protein 2; notch 2,

Search name

Mouse NOTCH2 ELISA KIT ,Mouse AGS2 ELISA KIT ,Mouse HJCYS ELISA KIT ,Mouse hN2 ELISA KIT ,Mouse Notch homolog 2 ELISA KIT ,Mouse neurogenic locus notch homolog protein 2 ELISA KIT ,Mouse notch 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse neurogenic locus notch homolog protein 2,NOTCH2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to NOTCH2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NOTCH2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NOTCH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NOTCH2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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