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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Semaphorin-7A, Sema7a含量
- 适应物种:
小鼠
- 标记物:
Semaphorin-7A, Sema7a
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Semaphorin- 7A, Sema7a ELISA KIT
Product Name:Mouse Semaphorin- 7A, Sema7a ELISA KIT
Packing:96T
Catalog No.:ELI-07283m
Gene Name:Mouse Sema7a
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Sema7a
Alternative Name:Mouse Semaphorin-7A, Sema7a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Semaphorin- 7A, Sema7a ELISA KIT allows for the in vitro quantitative determination of Mouse Sema7a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Semaphorin- 7A, Sema7a ELISA KIT has been pre-coated with an Mouse Semaphorin-7A, Sema7a antibody specific to Mouse Sema7a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sema7a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sema7a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sema7a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
BSA 不含有蛋白酶以及RNase酶活性,同时不含IgG,纯度等级更高,作为稀释液或封闭液应用于EIA和ELISA等试验中。如Jackson的001-000-16X系列以及EquiTech-Bio的BAH67(无蛋白酶/IgG,也不含RNase)系列产品。 诊断级BSA 诊断级牛血清白蛋白粉末是专门用于减少可能会产生背景干扰以及细胞培养体系抑制等因素,不含蛋白酶、生物负载以及IgG,具有很低活性的内毒素 (低于3.0 EU
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