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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Transferrin receptor protein 2, Tfr2含量
- 适应物种:
小鼠
- 标记物:
Transferrin receptor protein 2, Tfr2
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Transferrin receptor protein 2, Tfr2 ELISA KIT
Product Name:Mouse Transferrin receptor protein 2, Tfr2 ELISA KIT
Packing:96T
Catalog No.:ELI-07150m
Gene Name:Mouse Tfr2
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Tfr2
Alternative Name:Mouse Transferrin receptor protein 2, Tfr2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Transferrin receptor protein 2, Tfr2 ELISA KIT allows for the in vitro quantitative determination of Mouse Tfr2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Transferrin receptor protein 2, Tfr2 ELISA KIT has been pre-coated with an Mouse Transferrin receptor protein 2, Tfr2 antibody specific to Mouse Tfr2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tfr2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tfr2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tfr2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验ELISA-based assay for scatchard analysis of ligand-receptor interactions
transferrin to its receptors on mouse fibroblasts and yieldedK d values similar to those obtained with other published methods. The assay is sensitive, rapid, and also convenient, because aliquots of semi-intact cells can be stored frozen. The perforated
Receptor-Mediated Transport of Drugs Across the BBB
receptor-mediated transcytosis or transport (RMT) across the BBB in vivo. Certain peptidomimetic monoclonal antibodies (mAb) for insulin receptor or transferrin receptor can also cross the BBB via RMT on the endogenous receptors. These mAb can act
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
Figure 19.22.2 Protein content of individual sample fractions following LARC. Receptor complexes bound to ligand‐coated beads were purified by sucrose gradient ultracentrifugation and washed in 1× PBS (W
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