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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白L-lactate dehydrogenase A chain, Ldha含量
- 适应物种:
小鼠
- 标记物:
L-lactate dehydrogenase A chain, Ldha
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse L- lactate dehydrogenase A chain, Ldha ELISA KIT
Product Name:Mouse L- lactate dehydrogenase A chain, Ldha ELISA KIT
Packing:96T
Catalog No.:ELI-07038m
Gene Name:Mouse Ldha
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Ldha
Alternative Name:Mouse L-lactate dehydrogenase A chain, Ldha
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse L- lactate dehydrogenase A chain, Ldha ELISA KIT allows for the in vitro quantitative determination of Mouse Ldha concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse L- lactate dehydrogenase A chain, Ldha ELISA KIT has been pre-coated with an Mouse L-lactate dehydrogenase A chain, Ldha antibody specific to Mouse Ldha .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ldha and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ldha , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ldha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
1 fibers in immunofluorescence and with rat type 1 MHCs (myosin heavy chain)in Western blotting;the cell line was established in 1986 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse that had been immunized with purified myosin
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
(GAPD), mRNA 828 *NM_000291 Homo sapiens phosphoglycerate kinase 1 (PGK1), mRNA 2727 *NM_005566 Homo sapiens lactate dehydrogenase A (LDHA), mRNA 2105 *NM_002954 Homo sapiens ribosomal protein S27a (RPS27A), mRNA 4156 *NM_000981 Homo sapiens
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