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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Fibroblast growth factor 8, Fgf8含量
- 适应物种:
小鼠
- 标记物:
Fibroblast growth factor 8, Fgf8
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Fibroblast growth factor 8, Fgf8 ELISA KIT
Product Name:Mouse Fibroblast growth factor 8, Fgf8 ELISA KIT
Packing:96T
Catalog No.:ELI-06869m
Gene Name:Mouse Fgf8
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Fgf8
Alternative Name:Mouse Fibroblast growth factor 8, Fgf8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Fibroblast growth factor 8, Fgf8 ELISA KIT allows for the in vitro quantitative determination of Mouse Fgf8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Fibroblast growth factor 8, Fgf8 ELISA KIT has been pre-coated with an Mouse Fibroblast growth factor 8, Fgf8 antibody specific to Mouse Fgf8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Fgf8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Fgf8 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Fgf8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验ABSTRACT Fibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epithelial-mesenchymal interactions by means of regulating cell proliferation
指在组织培养中,除了氨基酸、维生素、葡萄糖以及无机盐等正常成分之外,其可以代替培养基血清高分子物质的而促进细胞生长的物质。与发育因子和增殖因子为同义词,但因在组织培养中是以细胞增殖为直接目的,所以称增殖因素较确切。生长因子多为广义的肽激素,有胰岛素、表皮生长因素(EGF)、成纤细胞生长因素(fibroblast gfowth factor,FGF)、血小板来源增殖因素(platelet-derivedgrowth factor,PDGF)以及生长
Regulator of Epidermal Growth Factor Signaling: Sprouty
Sprouty was first discovered through its downregulatory effect on fibroblast growth factor (FGF) receptor pathway during tracheal development. Sprouty expression is also induced by the epidermal growth factor receptor (EGFR) cascade
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