NAD-dependent deacetylase sirt

Mouse NAD- dependent deacetylase sirtuin- 3, Sirt3 ELISA KIT

Product Name:Mouse NAD- dependent deacetylase sirtuin- 3, Sirt3 ELISA KIT
Packing:96T

Catalog No.:ELI-06862m

Gene Name:Mouse Sirt3

Detect Range:78.1-5000pg/ml

Sensitivity:46.875pg/ml

Target Protein Name:Mouse Sirt3

Alternative Name:Mouse NAD-dependent deacetylase sirtuin-3, Sirt3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse NAD- dependent deacetylase sirtuin- 3, Sirt3 ELISA KIT allows for the in vitro quantitative determination of Mouse Sirt3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse NAD- dependent deacetylase sirtuin- 3, Sirt3 ELISA KIT has been pre-coated with an Mouse NAD-dependent deacetylase sirtuin-3, Sirt3 antibody specific to Mouse Sirt3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sirt3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sirt3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sirt3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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