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- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白NAD-dependent deacetylase sirtuin-3, Sirt3含量
- 适应物种:
小鼠
- 标记物:
NAD-dependent deacetylase sirtuin-3, Sirt3
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse NAD- dependent deacetylase sirtuin- 3, Sirt3 ELISA KIT
Product Name:Mouse NAD- dependent deacetylase sirtuin- 3, Sirt3 ELISA KIT
Packing:96T
Catalog No.:ELI-06862m
Gene Name:Mouse Sirt3
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Sirt3
Alternative Name:Mouse NAD-dependent deacetylase sirtuin-3, Sirt3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse NAD- dependent deacetylase sirtuin- 3, Sirt3 ELISA KIT allows for the in vitro quantitative determination of Mouse Sirt3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse NAD- dependent deacetylase sirtuin- 3, Sirt3 ELISA KIT has been pre-coated with an Mouse NAD-dependent deacetylase sirtuin-3, Sirt3 antibody specific to Mouse Sirt3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sirt3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sirt3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sirt3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Performing Bronchoalveolar Lavage in the Mouse
., and Pons, F. 2004. Dose‐dependent effects of endotoxins on allergen sensitization and challenge in the mouse. Clin. Exp. Allergy 34:1789‐1795. Delayre‐Orthez, C., Becker, J
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