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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Mitochondrial uncoupling protein 2, Ucp2含量
- 适应物种:
小鼠
- 标记物:
Mitochondrial uncoupling protein 2, Ucp2
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Mitochondrial uncoupling protein 2,UCP 2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
UCP2,uncoupling protein 2,BMIQ4; SLC25A8; UCPH; solute carrier family 25 member 8; uncoupling protein 2
Search name
Mouse UCP2 ELISA KIT ,Mouse uncoupling protein 2 ELISA KIT ,Mouse BMIQ4 ELISA KIT ,Mouse SLC25A8 ELISA KIT ,Mouse UCPH ELISA KIT ,Mouse solute carrier family 25 member 8 ELISA KIT ,Mouse uncoupling protein 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Uncoupling Protein 2, Mitochondrial,UCP-2. concentrations in serum, tissue homogenates and other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to UCP-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for UCP-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain UCP-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UCP-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Detection of Uncoupling Protein-2 (UCP2) As a Mitochondrial Modulator of Apoptosis
There is an increasing evidence that uncoupling protein-2 (UCP2), a recently identified molecular sensor and suppressor of mitochondrial reactive oxygen species (ROS), plays an important role in �regulating apoptosis in different cell systems
UCP1 orthologues and paralogues (UCP2, UCP3, plant UCP), comparative functional studies require a system with identical mitochondrial, cellular, and genetic backgrounds. In this chapter, the protocols for the ectopic expression of mouse UCP
线粒体荧光探针大全:TMRM,Mitotracker,JC-1
is reduced in Alzheimer's disease and their protein and nucleic acids are affected by reactive oxygen species, including nitric oxide 10 (Chapter 18). Molecular Probes has a range of mitochondrion-selective dyes with which to monitor mitochondrial
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