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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白T-lymphocyte activation antigen CD80, Cd80含量
- 适应物种:
小鼠
- 标记物:
T-lymphocyte activation antigen CD80, Cd80
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse T-lymphocyte activation antigen CD80, Cd80 ELISA试剂盒
Product Name: Mouse T-lymphocyte activation antigen CD80, Cd80 ELISA试剂盒
Product Name: Mouse T-lymphocyte activation antigen CD80, Cd80 ELISA试剂盒
Gene Name: Mouse T-lymphocyte activation antigen CD80, Cd80
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse T-lymphocyte activation antigen CD80, Cd80
Alternative Name: Mouse T-lymphocyte activation antigen CD80, Cd80
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse T-lymphocyte activation antigen CD80, Cd80 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse T-lymphocyte activation antigen CD80, Cd80 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse T-lymphocyte activation antigen CD80, Cd80 ELISA试剂盒 has been pre-coated with an Mouse T-lymphocyte activation antigen CD80, Cd80 antibody specific to Mouse T-lymphocyte activation antigen CD80, Cd80 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse T-lymphocyte activation antigen CD80, Cd80 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse T-lymphocyte activation antigen CD80, Cd80 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse T-lymphocyte activation antigen CD80, Cd80 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验CD80 分子 CD80 常用单克隆抗体或代号: B7- 1,BB1 主要表达细胞: Ba,Mac,TStr [B] 分子质量(kDa)和结构: gp60(IgSF) 功 能: 活化B细胞抗原,CD28、CTLA- 4配体,提供T细胞共刺激信号 CD80 Aka B7-1, BB1 Ligand for T cell marker CD28 or CTLA4 (CD152
Collection, Separation, and Activation of Human T Lymphocytes
(3 ). In particular, activation of antigen-specific CD4+ T cells of the Th2 subset in the lungs, resulting in interleukin (IL)-5 secretion, plays a major role in allergic inflammation of the airways (4 ). T-cell activation requires two signals, one provided
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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