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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Caspase-8, Casp8含量
- 适应物种:
小鼠
- 标记物:
Caspase-8, Casp8
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Caspase-8, Casp8 ELISA试剂盒
Product Name: Mouse Caspase-8, Casp8 ELISA试剂盒
Product Name: Mouse Caspase-8, Casp8 ELISA试剂盒
Gene Name: Mouse Caspase-8, Casp8
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Caspase-8, Casp8
Alternative Name: Mouse Caspase-8, Casp8
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Caspase-8, Casp8 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Caspase-8, Casp8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Caspase-8, Casp8 ELISA试剂盒 has been pre-coated with an Mouse Caspase-8, Casp8 antibody specific to Mouse Caspase-8, Casp8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Caspase-8, Casp8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Caspase-8, Casp8 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Caspase-8, Casp8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验3) 依赖性细胞死亡来介导中暑。 热应激通过热休克转录因子 1 (HSF1) 增加 ZBP1 的表达,并通过独立于核酸感应作用的机制激活 ZBP1。ZBP1、RIPK3 或混合谱系激酶域样 (MLKL) 和 caspase-8 的缺失降低了热应激诱导的循环衰竭、器官损伤和致死率。因此,ZBP1 似乎具有协调宿主对热应激反应的第二个功能。 临床上,中暑的特征是极度高热、全身炎症反应、循环衰竭、出血和凝血障碍,以及热相关细胞毒性、炎症和弥散性血管内凝血 (DIC) 之间复杂相互作用导致的多器官
凋亡的三岔路口,这个通路把控!韩家淮院士/莫玮等揭示坏死性凋亡的新机制
发育过程中 caspase-8 蛋白 Thr265 位点磷酸化抑制其对坏死性凋亡的阻断作用 既然在体外 Thr265 位点磷酸化能够逆转 caspase-8 对坏死性凋亡的阻断作用,那在体内是否也有相同的结果呢?作者构建了 Casp8T265E/T265E 组成性磷酸化突变小鼠,发现纯合小鼠卵黄囊血管系统异常,胚胎致死。免疫荧光和免疫组化结果显示 Casp8T265E/T265E 突变小鼠中坏死性凋亡的标志物 pRIP3 和 pMLKL 显著上调。与此同时,Rip3 和 Mlkl 缺陷能够逆转
、Caspase-8 抑制剂、对照细胞。本试剂盒的实验结果可与核小体ELISA 试剂盒(QIA25)、细胞死亡检测NMP ELISA 试剂盒的检测结果相互印证。 27、Caspase-8 Activity Assay Kit, HTS 目录号:HTS03 包装:100T/Kit 500T/Kit 1000T/Kit 本试剂盒提供了一种简单而快捷的高通量检测细胞裂解液中Caspase-8 活性的方法。本试剂盒适用于多种物种来源的Caspase-8 的检测。试剂盒包括所有必须的试剂
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