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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Microtubule-associated protein tau, Mapt含量
- 适应物种:
小鼠
- 标记物:
Microtubule-associated protein tau, Mapt
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Microtubule-associated protein tau, Mapt ELISA试剂盒
Product Name: Mouse Microtubule-associated protein tau, Mapt ELISA试剂盒
Product Name: Mouse Microtubule-associated protein tau, Mapt ELISA试剂盒
Gene Name: Mouse Microtubule-associated protein tau, Mapt
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Microtubule-associated protein tau, Mapt
Alternative Name: Mouse Microtubule-associated protein tau, Mapt
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Microtubule-associated protein tau, Mapt ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Microtubule-associated protein tau, Mapt concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Microtubule-associated protein tau, Mapt ELISA试剂盒 has been pre-coated with an Mouse Microtubule-associated protein tau, Mapt antibody specific to Mouse Microtubule-associated protein tau, Mapt .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Microtubule-associated protein tau, Mapt and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Microtubule-associated protein tau, Mapt , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Microtubule-associated protein tau, Mapt in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验of microtubule-associated proteins. Tau is strongly expressed in neurons, localized in the axon and is essential for neuronal architecture, plasticity and network. In animal models, overexpression of mutated Tau proteins is often used to induce a Tau pathology
Tau Phosphorylation Both In Vitro and in Cells
Tau was originally isolated from brain microtubules and shown to be a microtubule-associated protein (MAP) that promoted tubulin polymerization (1 ). It is largely confined to axons, where it is the major MAP. It promotes microtubule nucleation
Cell-Based Assays for Regulators of Tau Biology
, which may ultimately lead to translational outcomes for AD. In particular, the intracellular microtubule-associated protein tau that accumulates in AD is found in normal cells. Manipulating these cells may allow us to address basic questions about tau biology
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