- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-06346m
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Ectodysplasin-A, Eda含量
- 适应物种：
小鼠
- 标记物：
Ectodysplasin-A, Eda
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Ectodysplasin-A, Eda ELISA试剂盒
Product Name: Mouse Ectodysplasin-A, Eda ELISA试剂盒
Product Name: Mouse Ectodysplasin-A, Eda ELISA试剂盒
Gene Name: Mouse Ectodysplasin-A, Eda
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Ectodysplasin-A, Eda
Alternative Name: Mouse Ectodysplasin-A, Eda
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Ectodysplasin-A, Eda ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Ectodysplasin-A, Eda concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Ectodysplasin-A, Eda ELISA试剂盒 has been pre-coated with an Mouse Ectodysplasin-A, Eda antibody specific to Mouse Ectodysplasin-A, Eda .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ectodysplasin-A, Eda and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ectodysplasin-A, Eda , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ectodysplasin-A, Eda in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
到工作浓度，后面的显色效果才是最佳的! BSA主要是封闭液中用到，其实作用显而易见是起到封闭效果，就是结合包被过程中未结合的结合位点，提高ELISA中的特异性结合，提高实验的精确度。一般浓度是1%-2% 3、牛血清白蛋白BSA的等电点是多少 天然牛血清白蛋白(BSA)的等电点为4.6~5.8，经无水乙二胺(EDA)和碳化二亚胺(EDC)反应，改性后的BSA等电点为8.6。 4、牛血清蛋白(BSA)，牛血清蛋白(第五组分)之间有什么区别?
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