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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Protein S100-A9, S100a9含量
- 适应物种:
小鼠
- 标记物:
Protein S100-A9, S100a9
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Protein S100-A9, S100a9 ELISA试剂盒
Product Name: Mouse Protein S100-A9, S100a9 ELISA试剂盒
Product Name: Mouse Protein S100-A9, S100a9 ELISA试剂盒
Gene Name: Mouse Protein S100-A9, S100a9
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Protein S100-A9, S100a9
Alternative Name: Mouse Protein S100-A9, S100a9
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Protein S100-A9, S100a9 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Protein S100-A9, S100a9 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Protein S100-A9, S100a9 ELISA试剂盒 has been pre-coated with an Mouse Protein S100-A9, S100a9 antibody specific to Mouse Protein S100-A9, S100a9 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Protein S100-A9, S100a9 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Protein S100-A9, S100a9 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Protein S100-A9, S100a9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人S100A9 蛋白 ( S100A9 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 S100A9 单抗包被于酶标板上,标准品和样品中的 S100A9与单抗结合,加入生物素化的抗人
小鼠S100A9蛋白(S100A9)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠S100A9 蛋白 ( S100A9 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 S100A9 单抗包被于酶标板上,标准品和样品中的 S100A9与单抗结合,加入
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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