Mouse Growth /differentiation factor 2, GDF2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GDF2,BMP 9,BMP-9; BMP9; GDF-2; bone morphogenetic protein 9; growth/differentiation factor 2; growth differentiation factor 2
Search name
Mouse GDF2 ELISA KIT ,Mouse BMP 9 ELISA KIT ,Mouse BMP-9 ELISA KIT ,Mouse BMP9 ELISA KIT ,Mouse GDF-2 ELISA KIT ,Mouse bone morphogenetic protein 9 ELISA KIT ,Mouse growth/differentiation factor 2 ELISA KIT ,Mouse growth differentiation factor 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse growth/differentiation factor 2,GDF-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDF-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GDF-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GDF-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GDF-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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