Sodium-dependent dopamine tran
sporter, Slc6a3 ELISA KIT/ Mouse Sodium-dependent dopamine transporter, Slc6a3 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-05320m
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Sodium-dependent dopamine transporter, Slc6a3含量
- 适应物种：
小鼠
- 标记物：
Sodium-dependent dopamine transporter, Slc6a3
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Sodium-dependent dopamine transporter, Slc6a3 ELISA试剂盒
Product Name: Mouse Sodium-dependent dopamine transporter, Slc6a3 ELISA试剂盒
Product Name: Mouse Sodium-dependent dopamine transporter, Slc6a3 ELISA试剂盒
Gene Name: Mouse Sodium-dependent dopamine transporter, Slc6a3
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Sodium-dependent dopamine transporter, Slc6a3
Alternative Name: Mouse Sodium-dependent dopamine transporter, Slc6a3
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Sodium-dependent dopamine transporter, Slc6a3 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Sodium-dependent dopamine transporter, Slc6a3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Sodium-dependent dopamine transporter, Slc6a3 ELISA试剂盒 has been pre-coated with an Mouse Sodium-dependent dopamine transporter, Slc6a3 antibody specific to Mouse Sodium-dependent dopamine transporter, Slc6a3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sodium-dependent dopamine transporter, Slc6a3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sodium-dependent dopamine transporter, Slc6a3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sodium-dependent dopamine transporter, Slc6a3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
FA Extraction of [insoluble] Amyloid beta from Mouse Brain
at room temperature. 5. CAUTION: Sodium azide (NaN3 ) is highly toxic. 实验步骤 1. Mix 200 μl 10% (w/v) homogenate into 440 μl cold formic acid (minimum 95%, Sigma, F-0507) in a microcentrifuge tube. 2. Sonicate
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
H2O can also be used -> add 1.42g for 1x and 14.2g for 10x PBS. Make up to 1 litre with dd H2O. PBS wash: 100ml 1x PBS 10ul Tween (0.01%, final concentration) Citrate/Acetate Buffer pH6.0: 1. 500ml 0.1M Sodium Acetate Sodium acetate - 4.1g. dd H2O - 500ml
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